openarray real-time qpcr analysis software (v1.0.4)  (Thermo Fisher)

Journal: PLoS ONE

Article Title: MicroRNA Profiling of CSF Reveals Potential Biomarkers to Detect Alzheimer`s Disease

doi: 10.1371/journal.pone.0126423

Figure Lengend Snippet: Comparisons of 199 miRNAs assessed in OpenArray analysis of smallRNA isolated from CSF of patients with AD (n = 22) and controls (n = 28). The volcano plot displays the relationship between fold change and significance between the two groups, applying a student’s t-test. The y-axis depicts the negative log10 of p-values of the t-tests (the horizontal slider at 1.3 corresponds to a p-value of 0.05, a higher value indicates greater significance) and the x-axis is the difference in expression between the two experimental groups as log2 fold changes (vertical sliders indicate miRNAs as either up- or down regulated above a fold change of 1.5). Highlighted in green are the most reliable (6 abundant miRNAs from set A with RF≥0.8) and in red the most informative (9 less abundant miRNAs from set B above the critical MoR value d = 0.57) biomarkers according to the MoR-method (see Figs and ).

Article Snippet: Up to 3 resp. 12 samples per OpenArray plate were cycled simultaneously on a Biotrove OpenArray NT Cycler (Life Technologies) using OpenArray Real-Time qPCR Analysis Software (v1.0.4) with a pre-assigned cycling program to calculate quantification cycle (Cq) defined as the number of cycles at which the fluorescence signal is significantly above the threshold.

Techniques: Isolation, Expressing